Methanotroph Commons
Home
Getting Started
Wiki
Introduction
History
Methanotroph Taxonomy
Culturing Tips
Genetics
Genomes
Biochemistry
Metabolic Pathways
Performance and Yield
Forum
Announcements
Jobs and Positions
Questions and Discussion
General Questions
Lab Techniques
Blog
About
Log in
Register
Performance and Yield
Page
History
Edit
Text
Visual
<p><b>8. Performance and Yield Data</b></p> <p>Methanotrophs have been considered for use in industrial applications since the 1970s, and a variety of studies have assessed performance and yield data for these bacteria, including both gamma- and alpha-proteobacteria. Table 8.1 shows values reported for either theoretical predictions (<a title="Anthony, 1978 #10" href="/wiki/references#_ENREF_14">Anthony, 1978</a>, <a title="Anthony, 1982 #8" href="/wiki/references#_ENREF_15">1982</a>; <a title="Leak, 1986 #142" href="/wiki/references#_ENREF_117">Leak and Dalton, 1986b</a>)(Anthony, 1978, 1982; Leak & Dalton, 1986b) or experimental measurements (<a title="Harwood, 1972 #106" href="/wiki/references#_ENREF_88">Harwood and Pirt, 1972</a>; <a title="Lamb, 1980 #138" href="/wiki/references#_ENREF_115">Lamb and Garver, 1980</a>; <a title="Leak, 1986 #141" href="/wiki/references#_ENREF_116">Leak and Dalton, 1986a</a>; <a title="Park, 1991 #189" href="/wiki/references#_ENREF_140">Park et al., 1991</a>; <a title="Park, 1992 #192" href="/wiki/references#_ENREF_141">Park et al., 1992</a>). The values that have been obtained vary depending on which assimilation pathway was utilized, which form of the MMO was expressed, and which nitrogen source was used. Growth rates vary widely, depending on the strain.</p> <p> </p> <table width="637" border="1" cellspacing="0" cellpadding="0"> <tbody> <tr> <td valign="top" width="67"><b> </b></td> <td valign="top" width="62"><b> </b></td> <td colspan="4" valign="top" width="220"><b>Theoretical Predictions</b></td> <td colspan="5" valign="top" width="288"><b>Experimental Measurements</b></td> </tr> <tr> <td valign="top" width="67"><b>Strain Type</b></td> <td valign="top" width="62"><b>MMO</b></td> <td valign="top" width="50"><b>Y</b></td> <td valign="top" width="44"><b>% CO<sub>2</sub></b></td> <td valign="top" width="60"><b>CCE</b></td> <td valign="top" width="66"><b>% energy</b></td> <td valign="top" width="42"><b>Y</b></td> <td valign="top" width="60"><b>% CO<sub>2</sub></b></td> <td valign="top" width="42"><b>CCE</b></td> <td valign="top" width="72"><b>Biomass</b></td> <td valign="top" width="72"><b>μ</b><b><sup>-1</sup></b><b></b></td> </tr> <tr> <td valign="top" width="67">Gamma</td> <td valign="top" width="62">pMMO</td> <td valign="top" width="50">1.09</td> <td valign="top" width="44">31</td> <td valign="top" width="60">38-63</td> <td valign="top" width="66">35-63</td> <td valign="top" width="42">1.2</td> <td valign="top" width="60">33.5</td> <td valign="top" width="42">43.3</td> <td valign="top" width="72">20-25</td> <td valign="top" width="72">0.2-0.37</td> </tr> <tr> <td valign="top" width="67">Gamma</td> <td valign="top" width="62">sMMO</td> <td valign="top" width="50">0.75</td> <td valign="top" width="44">54</td> <td valign="top" width="60">32-42</td> <td valign="top" width="66">54-66</td> <td valign="top" width="42">0.8</td> <td valign="top" width="60">64</td> <td valign="top" width="42">31</td> <td valign="top" width="72">25-29</td> <td valign="top" width="72">0.02-0.2</td> </tr> <tr> <td valign="top" width="67">Alpha</td> <td valign="top" width="62">pMMO</td> <td valign="top" width="50">0.94</td> <td valign="top" width="44">42</td> <td valign="top" width="60">34-54</td> <td valign="top" width="66">34-80</td> <td valign="top" width="42">0.8</td> <td valign="top" width="60">-</td> <td valign="top" width="42">-</td> <td valign="top" width="72">6-20</td> <td valign="top" width="72">0.12</td> </tr> <tr> <td valign="top" width="67">Alpha</td> <td valign="top" width="62">sMMO</td> <td valign="top" width="50">0.65</td> <td valign="top" width="44">60</td> <td valign="top" width="60">36-39</td> <td valign="top" width="66">57-68</td> <td valign="top" width="42">0.22-0.5</td> <td valign="top" width="60">88</td> <td valign="top" width="42">-</td> <td valign="top" width="72">3</td> <td valign="top" width="72">0.013-0.08</td> </tr> </tbody> </table> <p>Y, g cell dry wt/g substrate; % CO<sub>2</sub>, % methane oxidized to CO<sub>2</sub>; CCE, carbon conversion efficiency (% CH<sub>4</sub> metabolized for biomass); % energy, % methane metabolized for energy; biomass, g wet wt/L fermentation broth; μ<sup>-1</sup>, specific growth rate.</p> <p> </p> <p>Although it may be predicted that the yields of methanotrophs should be higher using ammonium as a nitrogen source instead of nitrate, in fact, the reported yields are similar (<a title="Leak, 1986 #141" href="/wiki/references#_ENREF_116">Leak and Dalton, 1986a</a>).</p>
Cancel
Wiki Pages
Introduction
History
Methanotroph Taxonomy
General Culturing Tips
Genetics
Genomes
Biochemistry
Metabolic Pathways
Performance and Yield
Insert/edit link
Enter the destination URL
URL
Title
Open link in a new window/tab
Or link to existing content
Search
No search term specified. Showing recent items.
Cancel