Question: What is the best way to preserve methanotrophs in long-term storage?

The Methanotroph Commons Forums Questions and Discussion Lab Techniques Question: What is the best way to preserve methanotrophs in long-term storage?

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    Frances Chu
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    Answer:
    Traditionally, 10% dimethyl sulfoxide (DMSO) has been used for -80C long-term storage of methanotrophs. However, various labs have had success storing methanotrophs in other cryoprotectants such as glycerol, PEG, or trehalose. Other labs have placed cells on filter paper for -80C storage. Some have even lyophilized cultures for long-term storage. The rule of thumb is to find the protocol that works best in your hands and stick with that method. The following references discuss several methods for long-term preservation:

    Doronina, N. V.; Trotsenko, Y.A. 1992. Method for storage of methylotrophic and heterotrophic microorganisms. Prikladnaya Biokhimiya I Mikrobiologiya. 28(4): 631-635

    Hoefman, S., Van Hoorde, K., Boon, N., Vandamme, P., De Vos, P., and Heyle, K. 2012. Survival or Revival: Long-Term Preservation Induces a Reversible Viable but Non-Culturable State in Methane-Oxidizing Bacteria. 7(4): e34196.

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