Methanotroph Commons » All Posts http://methanotroph.org/forums/feed Sun, 12 Apr 2026 05:31:50 +0000 http://bbpress.org/?v=2.5.4-5380 en-US http://methanotroph.org/forums/topic/asm-2014-new-microbes-and-pathways-c1-metabolism/#post-333 <![CDATA[ASM 2014: New Microbes and Pathways-C1 Metabolism.]]> http://methanotroph.org/forums/topic/asm-2014-new-microbes-and-pathways-c1-metabolism/#post-333 Mon, 10 Feb 2014 22:59:39 +0000 Marina Kalyuzhnaya Join us in Boston at ASM 2014 for new discoveries in C1-world from natural methane cycle to CH4/CO based biocatalysis!
 

Session Title: New Microbes and Pathways-C1 Metabolism
Session Type: Symposium
Session Start/End Time: Sunday, May 18, 2014, 2:00 PM – 4:30 PM
Location: Meeting Room 102A
Description: Methane (CH4), the simplest organic molecule, is an essential component of the global carbon cycle. It is one of the most potent greenhouse gases. Today, methane is not only one of the major contributors for climate change it is the primary target for near-term climate regulation. Biological methane utilization (methanotrophy) is one of the major controls of the methane budget. Methanotrophy has been previously discussed in terms of specific functional metabolic modules that thought to be relatively well understood. Recent efforts in characterization of C1-cycles in aerobic and/or anaerobic environments uncover new metabolic arrangements for microbial methane utilization. These new discoveries continue to challenge our understanding of biochemistry, biology and evolution of methanotrophy as one of the core microbial functions. At the same time, they open-up new directions for methane mitigation and provide a multitude of potential applications for biotechnology. Carbon monoxide (CO) is one of the most important reactants in the troposphere, and contributes indirectly to radiative forcing. The dynamics of CO, like methane, are determined in part by bacteria in terrestrial and aquatic system. The distribution and diversity of CO oxidizers remain largely speculative. However, new observations suggest that they occur in extreme environments, in some cases as dominant members of the microbiota, and are represented by both Bacteria and Archaea. Results from analyses of halophilic CO oxidizers suggest that some representatives of the group could survive on contemporary Mars in surface or near-surface brines using CO as an energy source.
Convener
5/18/2014 2:00:00 PM
Marina Kalyuzhnaya;
Univ. of Washington, Seattle, WA.
Convener
5/18/2014 2:00:00 PM
Gary M. King;
Louisiana State Univ., Baton Rouge, LA.
Presentations:
Methane Cycle: A Fresh Look at an Old Story
5/18/2014 2:00:00 PM
Marina Kalyuzhnaya;
Univ. of Washington, Seattle, WA.
Comparative Genomics of Methanotrophs: Tales of Libertines, Teetotalers, and Recidivists
5/18/2014 3:00:00 PM
Peter F. Dunfield;
Univ. of Calgary, Calgary, Canada.
Thermophiles, Halophiles and Mars: Aerobic Carbon Monoxide Oxidizers Going to Extremes
5/18/2014 4:00:00 PM
Gary M. King;
Louisiana State Univ., Baton Rouge, LA.
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]]> http://methanotroph.org/forums/topic/question-what-is-the-best-way-to-get-rid-of-mold-from-oxoid-anaerobic-jars/#post-311 <![CDATA[Question: What is the best way to get rid of mold from Oxoid anaerobic jars?]]> http://methanotroph.org/forums/topic/question-what-is-the-best-way-to-get-rid-of-mold-from-oxoid-anaerobic-jars/#post-311 Wed, 05 Feb 2014 23:42:22 +0000 Frances Chu Answer: UV light can be used once per month for 15 min on the jars without affecting the jar’s integrity. To keep jars clean, always place plates in an open plastic bag before putting them into the jars. The plastic bags that are used to package sterile petri plates are great for this purpose.

]]> http://methanotroph.org/forums/topic/question-what-is-the-best-way-to-preserve-methanotrophs-in-long-term-storage/#post-310 <![CDATA[Question: What is the best way to preserve methanotrophs in long-term storage?]]> http://methanotroph.org/forums/topic/question-what-is-the-best-way-to-preserve-methanotrophs-in-long-term-storage/#post-310 Wed, 05 Feb 2014 23:39:43 +0000 Frances Chu Answer:
Traditionally, 10% dimethyl sulfoxide (DMSO) has been used for -80C long-term storage of methanotrophs. However, various labs have had success storing methanotrophs in other cryoprotectants such as glycerol, PEG, or trehalose. Other labs have placed cells on filter paper for -80C storage. Some have even lyophilized cultures for long-term storage. The rule of thumb is to find the protocol that works best in your hands and stick with that method. The following references discuss several methods for long-term preservation:

Doronina, N. V.; Trotsenko, Y.A. 1992. Method for storage of methylotrophic and heterotrophic microorganisms. Prikladnaya Biokhimiya I Mikrobiologiya. 28(4): 631-635

Hoefman, S., Van Hoorde, K., Boon, N., Vandamme, P., De Vos, P., and Heyle, K. 2012. Survival or Revival: Long-Term Preservation Induces a Reversible Viable but Non-Culturable State in Methane-Oxidizing Bacteria. 7(4): e34196.

]]> http://methanotroph.org/forums/topic/c1-grc/#post-309 <![CDATA[C1 GRC]]> http://methanotroph.org/forums/topic/c1-grc/#post-309 Tue, 04 Feb 2014 23:05:48 +0000 Mary Lidstrom Molecular Basis of Microbial One-Carbon Metabolism
Probing the Biology: Chemistry Interface from Molecules to Ecosystems
August 10-15 2014
Mount Holyoke College
South Hadley, MA

Gordon Research conference covering broad aspects of the microorganisms that utilized one-carbon compounds, including enzymology, metabolism, microbiology, microbial ecology, and biotechnology. Emphasis is on the molecular aspects of autotrophic microbes, methanogens, methylotrophs, methanotrophs, and acetogens.

Applications are now being accepted.

The Molecular Basis of Microbial One-Carbon Metabolism Gordon Research Conference will be held in conjunction with the Molecular Basis of Microbial One-Carbon Metabolism Gordon Research Seminar. Aug. 9-10 at Mount Holyoke College. Those interested in attending both meetings must submit an application for the GRS in addition to an application for the GRC.

]]> http://methanotroph.org/forums/topic/c1-grc-announcement/#post-306 <![CDATA[C1 GRC Announcement]]> http://methanotroph.org/forums/topic/c1-grc-announcement/#post-306 Tue, 04 Feb 2014 22:03:10 +0000 Mary Lidstrom Molecular Basis of Microbial One-Carbon Metabolism
Probing the Biology: Chemistry Interface from Molecules to Ecosystems
August 10-15 2014
Mount Holyoke College
South Hadley, MA

Gordon Research conference covering broad aspects of the microorganisms that utilized one-carbon compounds, including enzymology, metabolism, microbiology, microbial ecology, and biotechnology. Emphasis is on the molecular aspects of autotrophic microbes, methanogens, methylotrophs, methanotrophs, and acetogens.

Applications are now being accepted.

The Molecular Basis of Microbial One-Carbon Metabolism Gordon Research Conference will be held in conjunction with the Molecular Basis of Microbial One-Carbon Metabolism Gordon Research Seminar. Aug. 9-10 at Mount Holyoke College. Those interested in attending both meetings must submit an application for the GRS in addition to an application for the GRC.

]]> http://methanotroph.org/forums/topic/arpa-e-announces-new-remote-projects/#post-284 <![CDATA[Reply To: ARPA-E announces new REMOTE projects]]> http://methanotroph.org/forums/topic/arpa-e-announces-new-remote-projects/#post-284 Fri, 27 Sep 2013 03:50:07 +0000 Bo Hu 4 of 15 projects propose biological conversion of methanol/methane to butanol, but none of these involves M. extorquens AM1 according to the descriptions. Instead, they prefer transferring the methanol utilization pathway to an anaerobic organism for fermentation. It is interesting to speculate their strategies and host organism.

]]> http://methanotroph.org/forums/topic/arpa-e-announces-new-remote-projects/#post-255 <![CDATA[ARPA-E announces new REMOTE projects]]> http://methanotroph.org/forums/topic/arpa-e-announces-new-remote-projects/#post-255 Mon, 23 Sep 2013 22:52:53 +0000 Mary Lidstrom ARPA-E has announced $34M awarded to 15 projects in the Reducing Emissions using Methanotrophic Organisms for Transportation Energy (REMOTE) program.

http://www.arpa-e.energy.gov/sites/default/files/documents/files/METALS%26REMOTE_Project_Descriptions_091913.pdf

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